|
|
|
| Department of
Biochemistry and Molecular Biology |
|
| Postal address |
Edificio
Santiago Gascón, Campus del Cristo |
| Postal code |
33006
Oviedo, SPAIN |
| Phone |
(+34) 985 104211 / (+34) 985 103565 |
| Fax |
(+34) 985 106279 |
|
| |
| |
|
|
| |
| RESEARCH INTEREST |
• Molecular
characterization of transduction signaling pathways in eukaryotic cells
•
Structure-function
relationships and mechanisms of regulation in potassium channels
|
|
| |
| TECNOLOGY AND KNOWLEDGE OFFER |
|
• Isolation and characterization of DNA and RNA.
• Cloning,
site-directed mutagenesis and heterologous functional gene expression.
• Microinjection
and biochemical or electrophysiological detection of heterologously
expressed proteins in Xenopus laevis
oocytes.
•
Functional characterization of proteins (protein-protein interactions,
double hybrid techniques, inmunoprecipitation and inmunodetection,
etc).
•
Transient and stable transfection of cultured eukaryotic cells.
•
Patch-clamp recording of single channel and macroscopic ionic currents.
• Fluorimetric
recording of single cell pH and intracellular calcium levels.
• Imaging system for in vivo FRET measurements in eukaryotic cells.
• TIRF microscopy and
TIRF/FRET combination at 440, 488 and 514 nm (CFP, GFP and YFP).
|
|
|
